Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc Tris Glycine Transfer Buffer 10X (Transblotting Buffer 10X), 1000ML
Tris Glycine Transfer Buffer at a 1X concentration with the addition of 20% methanol is used for electrophoretic transfer of proteins from denaturing polyacrylamide gel to a solid membrane ¿ i.e. Western blotting or Tris-glycine gel electrophoresis.
Biotang Inc Tricine-buffered Saline (5X) pH 7.0, 250 ml
Tricine-buffered Saline (5X) pH 7.0, 250 ml.
Biotang Inc E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml
E. Coli Lysis Buffer (Tris-HCl 50 mM,1% Triton,EDTA 1 mM, Beta-mercaptoethanol 10 mM), 250 ml.
Research Products International Corp TRIS, Buffered Saline, 500 ML
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
Bioworld TBS Buffer, Low Salt (10X), 1 L
Product DescriptionTris-buffered saline (TBS) is low salt used for Western blot washing with a concentration of 10X. It contains 500 mM Tris, 1.5M NaCl and has a pH level of 7.4.TBS is isotonic and non-toxic, and is used to dilute substances. This buffer is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.
Bioworld Tris/Glycine/SDS Buffer for Resolve Gel (1X), 40 PK(s)
1X Tris/Glycine/SDS Buffer for Resolve GelBuffer System: Pre-weighed Powder mix in pouches(packs)1 pouch(pack)= 500 mL
Boston Bioproducts Inc EDTA (0.5 M, pH 7.4, pH Adjusted with KOH) - 250ml
EDTA [Ethylenediaminetetraacetic acid] solution is commonly used in various molecular biology and protein biochemistry applications that require the chelation of divalent metal cations, such as calcium (Ca2+) and magnesium (Mg2+). EDTA is used extensively as an anticoagulant in blood samples as it chelates the calcium present, preventing coagulation and preserving blood cell morphology. It is also used in cell lysis buffers. This solution is adjusted with KOH.
STEMCELL Technologies RoboSep™ Buffer 2, Size: 250 mL
RoboSep™ Buffer 2 is recommended for select EasySep™ cell separation protocols. Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Teknova 0.5M Tris, 1.5M Sodium Chloride, 0.5% Tween-20, pH 7.7. 500mL, Sterile.
0.5M Tris,1.5M Sodium Chloride,0.5% Tween-20
Electron Microscopy Sciences Tris (Hydroxymethyl) Aminomethane Free Base, Reagent 100 GR
CAS #77-86-1 C₄H₁₁NO₃ F.W. 121.14
Assay: >99.9%
White crystalline powder. A 40% (w/v) aqueous solution. Hydroxymethyl Aminomethane.
Grade: Reagent
Electron Microscopy Sciences Tris Maleate Buffer 0.2M, pH range 6.4-8.0 250 ML
Please specify pH when ordering.
pH: 6.4-8.0
Sigma Aldrich Fine Chemicals Biosciences Tyrodes Salts With sodium500ML
SODIUM Tyrodes Salts With sodium500ML
Quality Biological Inc TBE Buffer 10X, 4 Liters
TBE (TRIS Borate EDTA) is often used in procedures involving nucleic acids, the most common being electrophoresis. ¿ Tris-acid solutions are effective buffers for slightly basic conditions, which keep DNA deprotonated and soluble in water. ¿ Applications: TBE Buffer, 1X is used for DNA electrophoresis. ¿ TBE Buffer, 0.5X is used for electrophoresis onto DEAE cellulose membrane. ¿ TBE Buffer, 2X is used for polyacrylamide and agarose electrophoresis. ¿ TBE is also used for other nucleic acid electrophoresis procedures such as Northern and Southern blots. ¿ Storage Conditions: 15 to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
![Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60040-100.0.JPG-250.jpg)
Research Products International Corp Tris Base Ultra Pure [Tris (Hydroxymethyl) Aminomethane], 100 Grams
Tris Base Ultra Pure is a commonly used reagent and buffer component. Tris Base, Tris(hydroxymethyl)aminomethane, is a primary amine that acts as a buffering agent to maintain a stable pH in experimental procedures.
It is particularly effective in the pH range of 7.2 to 9.0, making it suitable for applications requiring slightly alkaline to mildly acidic pH conditions. Tris Base Ultra Pure is highly purified to remove impurities and contaminants, ensuring the highest quality for laboratory use.
The ultra-pure form is essential for applications where the presence of impurities can negatively impact experimental results. Tris Base Ultra Pure is commonly used in various molecular biology techniques such as DNA and RNA extraction, protein purification, and enzymatic assays.
It is also utilized in the preparation of electrophoresis buffers and PCR reactions.